MABE1807

Anti-NuMA1 Antibody, clone AD6-1

clone AD6-1, from mouse

• 동의어(들): Nuclear mitotic apparatus protein 1, NuMA protein1, Nuclear matrix protein-22, NMP-22, SP-H antigen
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: AD6-1, monoclonal
• 종 반응성: human, mouse
• 포장: antibody small pack of 25 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001273490
• UniProt 수납 번호: Q14980-1
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NUMA1(4926)

설명

일반 설명

Nuclear mitotic apparatus protein 1 (UniProt: Q14980; also known as NuMA protein, Nuclear matrix protein-22, NMP-22, SP-H antigen) is encoded by the NUMA1 (also known as NMP22, NUMA) gene (Gene ID: 4926) in human. NMP-22 is a homodimeric nuclear protein that is highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. It is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes. It is also considered to be essential or proper alignment of the mitotic spindle during asymmetric cell divisions. NMP-22 resides in the nuclear matrix during interphase and then dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association and it remains there until the anaphase onset. As mitosis progresses it re-associates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. NMP-22 is phosphorylated in the C-terminal tail during mitosis, most likely by CDK1 and phosphorylation increases its solubility and promotes association with dynein and subsequent translocation to the spindle poles. O-glycosylation during cytokinesis at sites identical or close to phosphorylation sites interferes with the phosphorylation status of NMP-22.

특이성

Clone AD6-1 detects NuMA1 in human and mouse cells. It targets an epitope within 181 amino acids from the C-terminal region.

면역원

A recombinant fragment corresponding to 181 amino acids from the C-terminal region of human Nuclear mitotic apparatus protein 1 (NuMA1).

애플리케이션

Anti-NuMA1, clone AD6-1 Antibody, Cat. No. MABE1807, is a highly specific mouse monoclonal antibody that targets NUMA1 and has been tested in Immunocytochemistry and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NUMA1 in 10 µg of NIH/3T3 cell lysate.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected NUMA1 in HepG2 cells.

Immunocytochemistry Analysis: A representative lot detected NUMA1 in Immunocytochemistry applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).

Western Blotting Analysis: A representative lot detected NUMA1 in Western Blotting applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NUMA1 in 10 µg of HeLa cell lysate.

표적 설명

~250 kDa observed; 238.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash