MABE1785

Anti-Ubiquitinated Histone H2B (Lys123) Antibody, clone 1B3F12/A9

clone 1B3F12/A9, from mouse

• 동의어(들): Histone H2B K123 Ub1, Histone H2B.2 UbK123
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: 1B3F12/A9, monoclonal
• application: ChIP; WB
• 종 반응성: yeast, human
• 기술: ChIP: suitable; western blot: suitable

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1B3F12/A9, monoclonal
• 종 반응성: yeast, human
• 포장: antibody small pack of 25 μg
• 기술: ChIP: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_009553.1; NP_010510.3
• UniProt 수납 번호: P02293; P02294
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: Saccharomyces cerevisiae ... Htb2(852284) ; human ... H2BC1(255626)

설명

일반 설명

Histone H2B.2 (UniProt: P02294) is encoded by the HTB2 (also known as H2B2) gene (Gene ID: 852284) in Saccharomyces cerevisiae (yeast). Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.

특이성

Clone 1B3F12/A9 detects K123 ubiquitinated histone H2B in human and yeast. It targets an epitope within 16 amino acids from the C-terminal region of ubiquitinated yeast Histone H2B.

면역원

Conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of yeast Histone H2B ubiquitinated at Lysine 123.

애플리케이션

Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Antibody, Cat. No. MABE1785, is a highly specific mouse monoclonal antibody, that targets ubiquitinated Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected Ubiquitinated Histone H2B (Lys123) in wild type whole cell yeast extracts and ubp8 knockout whole cell yeast extracts.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquitinated Histone H2B (Lys123) in H2BK123ub relative to H2B at the coding sequence of the SPA2 gene in budding yeast (relative to WT) (Courtesy of Fred van Leeuwen, Ph.D., Division of Gene Regulation B4, The Netherlands Cancer Institute Plesmanlaan 121, Amsterdam Netherlands).

품질

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Ubiquitinated Histone H2B (Lys123) in HeLa cell acid extract.

표적 설명

~23 kDa observed; 14.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash