MABC87

Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)

clone Ab 1, from mouse

• 동의어(들): CD148, DEP-1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, DEP1, HPTP eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: Ab 1, monoclonal
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001091973
• UniProt 수납 번호: Q12913
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PTPRJ(5795)

설명

일반 설명

CD148, also known as Density-enhanced phosphatase 1 (DEP-1), is a membrane bound phosphatase that dephosphorylates a large number of protein targets and kinases thus serving as a regulator of many signaling cascades. CD148/DEP-1 plays a role in cell adhesion, migration, proliferation and differentiation. CD148/DEP-1 also plays a role in macrophage migration, adhesion and spreading. CD148/DEP-1 is also central to T-cell receptor signaling and acts as a negative regulator. CD148/DEP-1 acts as a positive regulator of endothelial cell survival and enhances the barrier function of epithelial junctions during reassembly. CD148/DEP-1 is also a key regulator of platelet activation and thrombosis as well as a tumor suppressor. CD148/DEP-1 is localized at the cell membrane particularly at cell projections, ruffle membranes, and cell junctions and it is expressed in lymphocytes and epithelial cells and expression increases as cell density increases.

면역원

His-tagged recombinant protein corresponding to Human CD148/DEP-1.

애플리케이션

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Inhibition and Immunoprecipitation for the detection of CD148/DEP-1.

Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Raji, HEK293 +/-CD148 HA transfection, and A431D +/- CD148 transfection cell lysates.

Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Mouse kidney lysates (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected CD148/DEP-1 in Epithelial Cells of human prostate tissue.

Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in frozen mouse embryos. (Takahashi, T., et al., (2003) MOLECULAR AND CELLULAR BIOLOGY. 23(5):1817–1831).

Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in various mouse tissues such as kidney, cortex and cornea. (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Inhibition Assay: A representative lot of this antibody inhibited proliferation of HRMEC′s (Primary human renal microvascular endothelial cells) (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated CD148/DEP-1 from Huvec and RMEC cell lysates (Takahashi, T., et al., (1999) J. Am. Soc. Nephrol. 10: 2135–2145).

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD148/DEP-1 in 10 µg of HeLa cell lysate.

표적 설명

~260 kDa observed.
This protein is highly glycosylated and may run higher than the calculated molecular weight of 146 kDa (isoform 1) or 57 kDa (isoform 2). Uncharacterized bands may be observed in some lysates.

물리적 형태

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable