MABC586

Anti-Sulf-2 Antibody, clone 8G1

clone 8G1, from mouse

• 동의어(들): Extracellular sulfatase Sulf-2, hSulf-2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 8G1, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001155313
• UniProt 수납 번호: Q8IWU5
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SULF2(55959)

설명

일반 설명

Sulf-2 is an endoplasmic reticulum enzyme that is involved in sulfur metabolism which is important in bone, cartilage, and skeletal development. Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.

면역원

Recombinant protein corresponding to Human Sulf-2.

애플리케이션

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-Sulf-2 Antibody, clone 8G1 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sulf-2.

Western Blot Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cell lysates.

Immunocytochemistry Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1.

품질

Evaluated by Western Blotting in MCF-7 conditioned media.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sulf-2 in 10 µg of MCF-7 conditioned media.

표적 설명

~125 & 75 kDa observed.
Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable