MABC186-I

Anti-phospho-p62 (Ser403) Antibody, clone 4F6

clone 4F6, from rat

• 동의어(들): Ubiquitin-binding protein p62, p62, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• 클론: 4F6, monoclonal
• application: IHC; WB
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable; western blot: suitable
• citations: 2

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 4F6, monoclonal
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_003891.1
• UniProt 수납 번호: Q13501
• 배송 상태: ambient
• 타겟 번역 후 변형: phosphorylation (pSer403)
• 유전자 정보: human ... SQSTM1(8878)

설명

일반 설명

Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human Sequestosome is a multi-functional co-interacting protein with a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chains and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF- B in response to upstream signals. It also serves as an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.

특이성

Clone 4F6 recognizes p62/SQSTM1 phosphorylated at Ser403 in human and mouse.

면역원

KLH-conjugated linear peptide corresponding to a sequence surrounding pSer403 of human phospho p62/SQSTM1.

애플리케이션

Detect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.

Western Blotting Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Western Blot application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).

Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).

품질

Evaluated by Western Blotting in Neuro2a cells.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.

표적 설명

~60 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1