MABC171

Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1

clone HMab-1, from mouse

• 동의어(들): Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: HMab-1, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_005887
• UniProt 수납 번호: O75874
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IDH1(3417)

설명

일반 설명

Isocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in various types of glioma.

특이성

This antibody is specific for the R132H mutation of Isocitrate Dehydrogenase 1 (IDH1).

면역원

KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1).

애플리케이션

Detect Isocitrate Dehydrogenase 1 (IDH1)-R132H using this Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1 validated for use in Western Blotting, IHC, ICC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blot Analysis: A representative lot of this antibody detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in a panel of wild-type and mutated recombinant IDH1 proteins.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in anaplastic and diffuse astrocytoma tissues and in primary glioblastoma tissues (Kato, Y., et al. (2009). Biochem Biophys Res Commun. 390(3):547-51.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in transfected glioblastoma and transfected LN229 cells (Kaneko, M. K., et al. (2011). Biochem Biophys Res Commun. 406(4):608-613.).

품질

Evaluated by Western Blot in MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1).

Western Blot Analysis: A 1:500 dilution of this antibody detected MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1) in WB.

표적 설명

~90 kDa observed. The calculated molecular weight of Isocitrate Dehydrogenase 1 (IDH1) is 47 kDa However, a Western Blot was performed using an MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1). Because MBP has a molecular weight of ~42 kDa, a band was observed at ~90 kDa

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1)

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable