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Merck
모든 사진(1)

Key Documents

MABC1182

Sigma-Aldrich

Anti-Serpina3n Antibody, clone 13H5

clone 13H5, from hamster(Armenian)

동의어(들):

Serine protease inhibitor A3N

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

hamster (Armenian)

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

13H5, monoclonal

종 반응성

mouse

반응하면 안 됨

human

기술

immunofluorescence: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

Serine protease inhibitor A3N (UniProt: Q91WP6; also known as Serpin A3N) is encoded by the Serpina3n (also known as Spi2) gene (Gene ID: 20716) in murine species. SerpinA3N is a secreted protein of the serpin family. Serpins bind and inhibit specific serine proteases and several serine proteases are reported to be substrates for SerpinA3N in vitro. It is synthesized with a signal peptide of 20 amino acids that is cleaved to generate the mature protein. SerpinA3N is expressed at high levels in brain, heart, liver, lung, spleen, testis, and thymus. However, its expression is low in kidney, skeletal muscle, and bone marrow. Serpin A3N expression can also be induced by bacterial lipopolysaccharides. The reactive center loop (RCL) of as SerpinA3N extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable. SerpinA3N is shown to be up-regulated in the dorsal root ganglia after nerve injury and mice lacking SerpinA3N are reported to develop more neuropathic mechanical allodynia than wild-type (WT) mice. SerpinA3N is also shown to attenuate activated T cell-mediated neuronal death and reduce the severity of disease in experimental autoimmune encephalomyelitis (EAE). (Ref.: Vicuna, L et al. (2015) Nat. Med. 21(5):518-23).

특이성

Clone 13H5 specifically targets murine Serpina3n. It is predicted to react with both pro- and mature forms of Serpina3n. This clone targets a sequence within 24 amino acids from the C-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of murine Serpina3n.

애플리케이션

Detect Serpina3n using this armenian hamster monoclonal Anti-Serpina3n Antibody, clone 13H5, Cat. No. MABC1182. It has been tested for use in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected increased Serpin A3N/Serpina3n immunoreactivity in late metastasis stage lung tissue by fluorescent immunohistochemistry staining of paraformaldehyde-fixed frozen lung sections in a study employing the murine MMTV-PyMT breast-to-lung metastasis model (Sevenich, L., et al. (2014). Nat. Cell Biol. 16(9):876-888).
Research Category
Apoptosis & Cancer

품질

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Serpina3n in 10 µg of mouse brain tissue lysate.

표적 설명

~46 kDa observed; 46.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G purified
Purified armenian hamster monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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