추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
monoclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
포장
antibody small pack of 25 μg
기술
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... BAX(581)
일반 설명
Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.
특이성
Clone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.
면역원
KLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.
애플리케이션
Detect using this mouse monoclonal Anti-Bax, clone 6A7 Antibody, Cat. No. MABC1176-25UG, validated for use in Immunoprecipitation, Immunofluorescence and Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).
Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).
Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).
Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).
Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).
품질
Evaluated by Immunoprecipitation in THP-1 cell lysates.
Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.
Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.
물리적 형태
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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