MABC1132

Anti-PD-1 Antibody, clone G4

clone G4, from hamster(Armenian)

• 동의어(들): Programmed cell death protein 1, Protein PD-1, mPD-1, CD279
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: hamster (Armenian)
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: G4, monoclonal
• 종 반응성: mouse
• 포장: antibody small pack of 25 μg
• 기술: flow cytometry: suitable
• NCBI 수납 번호: NP_032824.1
• UniProt 수납 번호: Q02242
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Pdcd1(18566)

설명

일반 설명

Protein PD-1, mPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 18566) in murine species. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 21-169), a transmembrane domain (aa 170-190), and a cytoplasmic domain (aa 191-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain (aa 31-139). PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-1 lacks SH2- or SH3-binding motifs on its cytoplasmic tail, but contains the N-terminal sequence VDYGEL that forms an immunoreceptor tyrosine-based inhibition motif (V/I/LxYxxL), which recruits SH2 domain-containing phosphatases. The cytoplasmic tail also contains the C-terminal sequence TEYATI, which forms an immunoreceptor tyrosine-based switch motif (TxYxxL). PD-1 ligation is reported to inhibit the activation of T-cell receptor proximal kinases, which results in attenuation of Lck-mediated phosphorylation of ZAP-70 and initiation of downstream events. PD-1 is also reported to impair the activation of the MEK-ERK MAP kinase pathway by inhibiting activation of PLC- 1 and Ras.

특이성

Clone G4 is an Armenian Hamster monoclonal antibody that specifically detects PD-1 in murine cells.

면역원

Murine PD-1Ig fusion protein.

애플리케이션

Affects Function Analysis: A representative lot detected PD-1 in Affects Funtion applications (Dronca, R.S., et. al. (2016). JCI Insight. 1(6); Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Overacre-Delgoffe, A.E., et. al. (2017). Cell. 169(6):1130; Tsushima, F., et. al. (2006). Blood. 110(1):180-5).

Flow Cytometry Analysis: A representative lot detected PD-1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96).

Detects Programmed cell death protein 1 using this armenian hamster monoclonal Anti-PD-1, clone G4, Cat. No. MABC1132, testted for use in Flow Cytometry and Affects Function.

품질

Evaluated by Flow Cytometry in EL4 T lymphoma cells.

Flow Cytometry Analysis: 1 µg of this antibody detected PD-1 in 1X10E6 EL4 T lymphoma cells.

표적 설명

~31.84 kDa calculated.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.