추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
3.91D, monoclonal
분자량
calculated mol wt 31.89 kDa
observed mol wt ~50 kDa
정제법
using protein G
종 반응성
virus
포장
antibody small pack of 100 μL
기술
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
동형
IgG3
에피토프 서열
N-terminal half
단백질 ID 수납 번호
UniProt 수납 번호
배송 상태
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
vaccinia virus ... poly> POLY(912267)
일반 설명
Genome polyprotein (UniProt: P14335) is encoded by the POLY gene (Gene ID: 912267) in West Nile virus. West Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. The envelope glycoprotein (E) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. The E protein (aa 291-791) contains a three-domain structure where domain I is an eight-stranded β-barrel in the center portion of the protein (aa 1-51, 134-195, and 284-297). The DII domain consists of 170 residues inserted into DI (aa 52-133 and 196-283). At the distal end of DII domain is a fusion loop (aa 98-110) and a conserved glycine-rich, hydrophobic sequence, which is important for flaviviral fusion. The third domain, DIII, is an Ig-like domain with seven β-strand and has been implicated in receptor binding. The E protein also has a single N-linked glycosylation site at Asn 154, located within the ectodomain of prM domain and is cleaved by cellular enzymes during viral particle maturation. Clone E60 specifically binds to the DII fusion loop and displays limited neutralizing activity and even at higher doses neutralization activity is shown to be between 26 and 68%. (Ref.: Martín-Acebes MA, et al. (2012). World J Virol. 1(2); 51-70).
특이성
Clone 3.91D is a mouse monoclonal antibody that detects West Nile virus envelope protein. It targets an epitope within the N-terminal half of polyprotein.
면역원
West Nile Virus (WNV; subtype KUNV) isolate OR393.
애플리케이션
Qualtiy Control Testing
Evaluated by Western Blotting with recombinant West Nile Virus envelope protein.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant West Nile Virus envelope protein.
Tested Applications
Western Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Western Blotting applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Saiyasombat, R., et al. (2014). Virol J.;11:150; Blitvich, B.J., et al. (2016). Am J Trop Med Hyg.;95(5):1185-1191).
ELISA Analysis: Various dilutions of this antibody detected recombinant West Nile Virus/Kunjin, Envelope protein.
Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Immunofluorescence applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Osorio, J.E., et al. (2012). Am J Trop Med Hyg.;87(3):565-72).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant West Nile Virus envelope protein.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant West Nile Virus envelope protein.
Tested Applications
Western Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Western Blotting applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Saiyasombat, R., et al. (2014). Virol J.;11:150; Blitvich, B.J., et al. (2016). Am J Trop Med Hyg.;95(5):1185-1191).
ELISA Analysis: Various dilutions of this antibody detected recombinant West Nile Virus/Kunjin, Envelope protein.
Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Immunofluorescence applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Osorio, J.E., et al. (2012). Am J Trop Med Hyg.;87(3):565-72).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-West Nile Virus/Kunjin, Envelope, clone 3.91D, Cat. No. MAB8151-I, is a mouse monoclonal antibody that detects envelope protein in West Nile virus and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
물리적 형태
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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