추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
2A8.2, monoclonal
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG2b
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NOS1(4842)
일반 설명
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
특이성
Reacts with nitrotyrosine.
면역원
Nitrated KLH.
애플리케이션
Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.
Immunohistochemistry:
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
품질
Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8ºC from date of receipt.
분석 메모
Control
Nitrated BSA
Nitrated BSA
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry.
Mihaela Dr Gu?anu,Brindu?a-Alina Petre,Michael Przybylski
Journal of Peptide Science null
A Kiss et al.
British journal of pharmacology, 155(7), 1015-1024 (2008-10-11)
Exogenous peroxynitrite from nanomolar to micromolar concentrations exerts cardioprotection. Here, we have assessed its effects on ischaemia- and reperfusion-induced ventricular arrhythmias in vivo and a possible role for mitochondrial K(ATP) channels in these effects, using the channel inhibitor 5-hydroxydecanoate (5-HD).
Ben C Creelan et al.
OncoTargets and therapy, 10, 4239-4250 (2017-09-19)
Omaveloxolone is a semisynthetic oleanane triterpenoid that potently activates Nrf2 with subsequent antioxidant function. We conducted a first-in-human Phase I clinical trial (NCT02029729) with the primary objectives to determine the appropriate dose for Phase II studies, characterize pharmacokinetic and pharmacodynamic
When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.
Petre, BA; Ulrich, M; Stumbaum, M; Bernevic, B; Moise, A; Doring, G; Przybylski, M
Journal of the American Society For Mass Spectrometry null
Deficiency of the Bax gene attenuates denervation-induced apoptosis.
P M Siu, S E Alway, P M Siu, S E Alway
Apoptosis null
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