추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
324, monoclonal
종 반응성
mouse, rat
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... L1CAM(3897) , VCAM1(7412)
일반 설명
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
특이성
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
면역원
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
애플리케이션
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
표적 설명
120-140 kDa
물리적 형태
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum.
Lindner, J, et al.
Nature, 305, 427-430 (1983)
Absence of layer-specific cadherin expression profiles in the neocortex of the reeler mutant mouse.
Hertel, N; Redies, C
Cerebral Cortex (1991)
Heparan sulfate sugar modifications mediate the functions of slits and other factors needed for mouse forebrain commissure development.
Conway, CD; Howe, KM; Nettleton, NK; Price, DJ; Mason, JO; Pratt, T
The Journal of Neuroscience null
Expression of ABCA2 protein in both non-myelin-forming and myelin-forming Schwann cells in the rodent peripheral nerve.
Takashi Saito, Katsuya Yamada, Yan Wang, Yukiko Tanaka, Kazuo Ohtomo, Kazuo Ishikawa, Nobuya Inagaki
Neuroscience Letters null
Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
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