MAB374-AF488

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate

clone 6C5, from mouse, ALEXA FLUOR^™ 488

• 동의어(들): Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 488
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 6C5, monoclonal
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): cat (based on 100% sequence homology), fish (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_001082253.1
• UniProt 수납 번호: P46406
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rabbit ... Gapdh(100009074)

설명

일반 설명

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

특이성

It has been reported that this antibody does not react with GAPDH from E. coli.

애플리케이션

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.

표적 설명

~38 kDa observed

물리적 형태

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable