MAB353B

Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

clone rat-401, from mouse, biotin conjugate

• 동의어(들): rat401, NES, Nbla00170, FLJ21841
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: biotin conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: rat-401, monoclonal
• 종 반응성: mouse, rat
• 반응하면 안 됨: human
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_037119
• UniProt 수납 번호: P21263
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

설명

일반 설명

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

특이성

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

면역원

Embryonic rat spinal cord

애플리케이션

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Developmental Neuroscience

Neural Stem Cells

품질

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

표적 설명

200-220 kDa calculated

물리적 형태

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

저장 및 안정성

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

분석 메모

Control
Mouse cortical neural stem cells (SCR029)

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable