MAB353A4

Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

clone rat-401, from mouse, ALEXA FLUOR^™ 488

• 동의어(들): rat401, NES, Nbla00170, FLJ21841
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 488
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: rat-401, monoclonal
• 종 반응성: rat, mouse
• 반응하면 안 됨: human
• 기술: immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_037119
• UniProt 수납 번호: P21263
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

설명

일반 설명

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

특이성

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

면역원

Embryonic rat spinal cord

애플리케이션

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

품질

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

표적 설명

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

물리적 형태

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

저장 및 안정성

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

분석 메모

Control
Mouse cortical neural stem cells (SCR029)

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable