추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
W27, monoclonal
종 반응성
bovine, human, monkey
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... HSPA4(3308)
특이성
Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
면역원
HSP70 from HeLa cells.
애플리케이션
Immunoblotting: 0.5-1 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.
물리적 형태
Format: Purified
분석 메모
Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A physiologically relevant atherogenic diet causes severe endothelial dysfunction within 4 weeks in rabbit.
Rai S, Hare DL, Zulli A
International Journal of Experimental Pathology null
Chi Lam Au Yeung et al.
Nature communications, 7, 11150-11150 (2016-03-30)
Advanced ovarian cancer usually spreads to the visceral adipose tissue of the omentum. However, the omental stromal cell-derived molecular determinants that modulate ovarian cancer growth have not been characterized. Here, using next-generation sequencing technology, we identify significantly higher levels of
High dietary taurine reduces apoptosis and atherosclerosis in the left main coronary artery: association with reduced CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia.
Zulli, A; Lau, E; Wijaya, BP; Jin, X; Sutarga, K; Schwartz, GD; Learmont, J; Wookey et al.
Hypertension null
Elisa Teyssou et al.
Neurobiology of aging, 58, 239-239 (2017-07-19)
Mutations in UBQLN2 have been associated with rare cases of X-linked juvenile and adult forms of amyotrophic lateral sclerosis (ALS) and ALS linked to frontotemporal dementia (FTD). Here, we report 1 known (c.1489C>T, p.Pro497Ser, P497S) and 3 novel (c.1481C>T, p.Pro494Leu
Jonathan Rios-Doria et al.
The Prostate, 63(3), 259-268 (2004-11-13)
E-cadherin, a fundamental component of the adherens junction, is known to mediate aggregation-dependent cell survival. We have previously identified a novel, calpain-dependent proteolytic cleavage of E-cadherin that resulted in the generation of a stable 100-kDa E-cadherin fragment (E-cad(100)) in prostate
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