MAB3317

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7

clone 113-5B7, Chemicon^®, from mouse

• 동의어(들): MT1-MMP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 113-5B7, monoclonal
• 종 반응성: rat, human, bovine, mouse
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG3κ
• NCBI 수납 번호: NM_004995.2
• UniProt 수납 번호: P50281
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MMP14(4323)

설명

특이성

Reacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.

면역원

CDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP

Epitope: a.a. 319-333

애플리케이션

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western Blot: 10 μg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.


When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.


Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.


Immunoprecipitation


EIA


Optimal working dilutions must be determined by the end user.

표적 설명

~59-69 kDa

물리적 형태

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.

저장 및 안정성

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Breast carcinoma tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Manufactured by Daiichi Fine Chemical Co., Ltd

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable