추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1J5, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt ~65.89 kDa
observed mol wt ~55 kDa
종 반응성
mouse, human
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1J5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Matrix metalloproteinase-14 (MMP-14). It targets an epitope within 16 amino acids from the extracellular domain.
면역원
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human Matrix metalloproteinase-14 (MMP14).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in L929 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MMP-14 in L929 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MMP-14 in A431 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound MMP14 peptides with a KD of 1.8 x 10-6 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected MMP-14 in one million HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MMP-14 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MMP-14 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in L929 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MMP-14 in L929 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MMP-14 in A431 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound MMP14 peptides with a KD of 1.8 x 10-6 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected MMP-14 in one million HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MMP-14 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MMP-14 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MMP-14, clone 1J5 ZooMAb®, Cat. No. ZRB1760, is a recombinant Rabbit monoclonal antibody that specifically targets MMP-14 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
표적 설명
Matrix metalloproteinase-14 (UniProt: P50281; also known as EC:3.4.24.80, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP) is encoded by the MMP14 gene (Gene ID: 4323) in human. MMPs are a family zinc-dependent endoproteinases that degrade collagen and other structural proteins and are responsible for much of the turnover of matrix components. Each MMP contains a protease domain with a conserved HExGHxxGxxHS/T sequence, where three histidine residues form a complex with a catalytic Zn atom. A regulatory domain with a conserved PRCGxPD motif helps to maintain MMPs in their latent form by binding of the cysteine residue to the active site Zn. MMP-14 is a single-pass type I membrane protein that is expressed in stromal cells of colon, breast, head, and neck. It is a membrane type matrix metalloproteinase (MT-MMP) that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-111) that are subsequently cleaved off to generate the active mature form. The propeptide maintains it in a latent state. The mature enzyme contains an extracellular domain (aa 112-541), a transmembrane domain (aa 542-562), and a cytoplasmic domain (aa 563-582). MMP14 contains four hemopexin domains within its extracellular domain and a cysteine switch (aa 91-98). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the release of propeptide activates the enzyme. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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