추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
클론
R4A, monoclonal
종 반응성
pig, chicken, monkey, canine, feline, human, hamster
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... SMTN(6525)
일반 설명
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
특이성
MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
면역원
Cytoskeletal extract of chicken gizzard.
애플리케이션
Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
Western blot
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
물리적 형태
Format: Purified
Liquid in buffer (PBS) with 0.1% sodium azide.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Yan Zhang et al.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, 19(6), e233-e240 (2010-01-23)
We have recently shown that native murine ventricular fibroblasts express both connexin43 (Cx43) and Cx45, and that the level of Cx43 expression influences intercellular coupling and cell proliferation. Relatively little is known, however, about how myocardial infarction (MI) influences expression
Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype.
Hector F Simosa, Grace Wang, XinXin Sui, Timothy Peterson, Vinod Narra, Dario C Altieri et al.
Journal of Vascular Surgery null
Eric van der Veer et al.
Circulation research, 97(1), 25-34 (2005-06-11)
Conversion of vascular smooth muscle cells (SMCs) from a proliferative state to a nonproliferative, contractile state confers vasomotor function to developing and remodeling blood vessels. Using a maturation-competent human SMC line, we determined that this shift in phenotype was accompanied
Sachiko Matsuzaki et al.
Human reproduction (Oxford, England), 31(3), 541-553 (2016-01-15)
Can deep infiltrating endometriotic stromal cells (DES) sense changes in extracellular matrix (ECM) stiffness and respond to them? Soft matrices inhibit cell proliferation and inactivate the fibrotic phenotype of DES in vitro. Deep infiltrating endometriosis (DIE) is characterized histologically by
Philippe Gomis et al.
Journal of neurosurgery, 102(4), 715-720 (2005-05-06)
The aim of this project was to study the perturbations of four smooth-muscle proteins and an extracellular protein, type I collagen, after subarachnoid hemorrhage (SAH) and to examine the possible preventive effects of dexamethasone. Using a one-hemorrhage rabbit model, the
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