추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
IIB5, monoclonal
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
동형
IgG1
배송 상태
wet ice
타겟 번역 후 변형
unmodified
일반 설명
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
특이성
Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
면역원
A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
애플리케이션
Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
물리적 형태
Format: Purified
Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
Protein A purified
저장 및 안정성
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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