추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
saturated ammonium sulfate (SAS) precipitated
항체 생산 유형
primary antibodies
클론
A2B5-105, monoclonal
종 반응성(상동성에 의해 예측)
mammals
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
동형
IgM
배송 상태
dry ice
타겟 번역 후 변형
unmodified
일반 설명
A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.
특이성
Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:
Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
면역원
Embryonic chicken retinal cells
애플리케이션
Anti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH.
Immunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Optimal working dilutions must be determined by end user.
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
물리적 형태
Ammonium Sulfate Precipitation
Format: Purified
Liquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA.
저장 및 안정성
Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.
분석 메모
Control
Type II astrocytes, human neural progenitors
Type II astrocytes, human neural progenitors
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
J Smith et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(18), 10032-10037 (2000-08-16)
We have discovered that intracellular redox state appears to be a necessary and sufficient modulator of the balance between self-renewal and differentiation in dividing oligodendrocyte-type-2 astrocyte progenitor cells. The intracellular redox state of freshly isolated progenitors allows prospective isolation of
A2B5 lineages of human astrocytic tumors and their recurrence.
Xia, Chun-Lin, et al.
International journal of oncology, 23, 353-361 (2003)
Thyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis.
Calza, L; Fernandez, M; Giuliani, A; Aloe, L; Giardino, L
Proceedings of the National Academy of Sciences of the USA null
Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma.
Rebetz, J; Tian, D; Persson, A; Widegren, B; Salford, LG; Englund, E; Gisselsson, D; Fan, X
Testing null
Ganglioside markers GD3, GD2, and A2B5 in fetal human neurons and glial cells in culture.
Satoh, J and Kim, S U
Developmental Neuroscience, 17, 137-148 (1995)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.