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Merck
모든 사진(1)

주요 문서

MAB308

Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2

ascites fluid, clone 4F10.2, Chemicon®

동의어(들):

DBH

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

4F10.2, monoclonal

종 반응성

rat, bovine, sheep

제조업체/상표

Chemicon®

기술

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

bovine ... Dbh(280758)
rat ... Dbh(25699)

일반 설명

Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.

특이성

Dopamine beta hydroxylase (DBH).

면역원

Purified bovine DBH.

애플리케이션

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.
Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.

Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

품질

Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

결합

Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG

물리적 형태

Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

R N Ranson et al.
Neuroscience, 141(4), 1935-1949 (2006-06-20)
Preganglionic neurones in the lumbosacral spinal cord give rise to nerves providing the parasympathetic and sympathetic innervation of pelvic organs. These neurones are modulated by neurotransmitters released both from descending supra-spinal pathways and spinal interneurones. Though serotonin has been identified
S Gutierrez et al.
Neuroscience, 228, 301-308 (2012-10-30)
Tissue and nerve damage can result in chronic pain. Yet, chronic pain after cesarean delivery is remarkably rare in women and hypersensitivity from peripheral nerve injury in rats resolves rapidly if the injury occurs in the puerperium. Little is known
Distribution and phenotype of Phox2a-containing neurons in the adult sprague-dawley rat.
Card JP, Lois J, Sved AF
The Journal of Comparative Neurology null
Glucocorticoid enhancement of memory requires arousal-induced noradrenergic activation in the basolateral amygdala.
Roozendaal, B; Okuda, S; Van der Zee, EA; McGaugh, JL
Proceedings of the National Academy of Sciences of the USA null
Y Zhang et al.
Neuroscience, 169(3), 1421-1437 (2010-05-19)
Taste buds signal the presence of chemical stimuli in the oral cavity to the central nervous system using both early transduction mechanisms, which allow single cells to be depolarized via receptor-mediated signaling pathways, and late transduction mechanisms, which involve extensive

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