추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
mouse, monkey, rat, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... DRD2(1813)
mouse ... Drd2(13489)
rat ... Drd2(24318)
rhesus monkey ... Drd2(694181)
일반 설명
The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.
특이성
Specific for Dopamine Receptor D2 short and long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (2). The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5).
면역원
A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic
loop #3.
loop #3.
애플리케이션
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Dopamine D2 Receptor Antibody is validated for use in ELISA, IH, IP, WB for the detection of Dopamine D2 Receptor.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.
Immunohistochemistry:
2-10 μg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.
Immunoprecipitation:
1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).
ELISA:
0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.
Optimal working dilutions must be determined by the end user.
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.
Immunohistochemistry:
2-10 μg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.
Immunoprecipitation:
1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).
ELISA:
0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.
Optimal working dilutions must be determined by the end user.
품질
Routinely evaluated by Western Blot on human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates.
표적 설명
50 kDa
물리적 형태
ImmunoAffinity Purified
Purified rabbit polyclonal liquid in PBS with 0.1% BSA.
저장 및 안정성
Stable for 1 year at -20ºC from date of receipt.
분석 메모
Control
Monkey brain tissue, rat brain lysate
Monkey brain tissue, rat brain lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Preweaning Mn exposure leads to prolonged astrocyte activation and lasting effects on the dopaminergic system in adult male rats.
Kern CH, Smith DR.
Synapse null
Evidence for differential cortical input to direct pathway versus indirect pathway striatal projection neurons in rats.
Lei, W; Jiao, Y; Del Mar, N; Reiner, A
The Journal of Neuroscience null
Presence of nicotinic, purinergic and dopaminergic receptors and the TASK-1 K+-channel in the mouse carotid body.
K?hlin J, Eriksson LI, Ebberyd A, Fagerlund MJ
Respiratory Physiology & Neurobiology null
Ultrastructural immunocytochemical localization of the dopamine D2 receptor and tyrosine hydroxylase in the rat ventral pallidum.
Mengual, Elisa and Pickel, Virginia M
Synapse, 43, 151-162 (2002)
Dopamine synthesis and D3 receptor activation in pancreatic ?-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations.
Ustione, A; Piston, DW
Molecular Endocrinology null
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