추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
10B8, monoclonal
종 반응성
mouse, bovine, human, chicken
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ELN(2006)
특이성
Recognizes human Elastin. The antibody is also reactive with elastin from chicken, and bovine and mouse. Antibody specificity has been determined by immunoblotting of purified chick tropoelastin and immunoprecipitation of tritium-labeled chick tropoelastin from extracts of chick aorta organ cultures.
면역원
Tropoelastin from 17-day old lathyric chick embryo aortae, extracted an purified according to the method of Rich and Foster (1982) Meth. Enzymol. 82: 665-673.
애플리케이션
Anti-Elastin Antibody, clone 10B8 detects level of Elastin & has been published & validated for use in ELISA, IF, IP & WB.
Immunoblotting
Immunofluorescence
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunofluorescence
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
표적 설명
66 kDa
물리적 형태
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
저장 및 안정성
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Skin
Skin
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Thomas J Kean et al.
Connective tissue research, 57(4), 319-333 (2016-04-30)
To determine the effect of reduced (5%) oxygen tension on chondrogenesis of auricular-derived chondrocytes. Currently, many cell and tissue culture experiments are performed at 20% oxygen with 5% carbon dioxide. Few cells in the body are subjected to this supra-physiological
Fumiaki Sato et al.
Clinical biochemistry, 39(7), 746-753 (2006-05-13)
It is known that elastin mRNA is transcribed from a single gene. The variety of tropoelastin isoforms results from multiple alternative splicing of the primary transcript. The purpose of this study was to investigate the characteristics of elastic fiber assembled
Elastin exhibits a distinctive temporal and spatial pattern of distribution in the developing chick limb in association with the establishment of the cartilaginous skeleton.
Hurle, J M, et al.
Journal of Cell Science, 107 ( Pt 9), 2623-2634 (1994)
Long-term stability and safety of transgenic cultured epidermal stem cells in gene therapy of junctional epidermolysis bullosa.
De Rosa, L; Carulli, S; Cocchiarella, F; Quaglino, D; Enzo, E; Franchini, E; Giannetti et al.
Stem Cell Reports null
Qirui Hu et al.
Matrix biology : journal of the International Society for Matrix Biology, 29(7), 621-628 (2010-07-06)
Heterozygous elastin gene mutations cause autosomal dominant cutis laxa associated with emphysema and aortic aneurysms. To investigate the molecular mechanisms leading to cutis laxa in vivo, we generated transgenic mice by pronuclear injection of minigenes encoding normal human tropoelastin (WT)
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