MAB1992

Anti-Integrin α3β1 Antibody, clone M-KID2

clone M-KID2, Chemicon^®, from mouse

• 동의어(들): VLA-3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: M-KID2, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; radioimmunoassay: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002204.1; NM_005501.1
• UniProt 수납 번호: P26006
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGA3(3675)

설명

일반 설명

Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, VLA-2, VL-3, VLA-4, VLA-5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha-3/beta-1) is a cell surface receptor that binds to fibronectin, laminin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells.

특이성

Recognizes an epitope related to the VLA-3 complex (alpha-3/beta-1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of ³⁵S-methionine labeled KJ29 cells.

면역원

Human renal carcinoma cell line (KJ29).

애플리케이션

Immunohistochemistry (works on Acetone-fixed paraffin-embedded tissues)

ELISA/RIA

Immunoprecipitation. When immunoprecipitated samples are run on SDS-PAGE under a) non-reducing conditions, two bands will be detected with an apparent molecular weight of 110 and 150kDa; and b) reducing (2% B-ME) conditions, a single molecular species of 130kDa is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight.

Optimal working dilutions must be determined by end user.

Anti-Integrin α3β1 Antibody, clone M-KID2 detects level of Integrin α3β1 & has been published & validated for use in ELISA, IP, RIA, IC, IH(P).

물리적 형태

Format: Purified

Immunoglobulin purified by Protein A. Purified immunoglobulin in PBS and 0.05% sodium azide as preservative.

저장 및 안정성

Maintain at 2°-8°C in undiluted aliquots for up to 12 months from date of receipt.

분석 메모

Control
Cell line: KJ29 renal carcinoma adherent cells.
Tissue: Kidney; antibody reacts with basal layer podocytes and basement membrane of distal tubules.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable