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Key Documents

MAB1615

Sigma-Aldrich

Anti-Neurofilament 70 kDa Antibody, clone DA2

culture supernatant, clone DA2, Chemicon®

동의어(들):

Anti-CMT1F, Anti-CMTDIG, Anti-NF-L, Anti-NF68, Anti-NFL, Anti-PPP1R110

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

culture supernatant

항체 생산 유형

primary antibodies

클론

DA2, monoclonal

종 반응성

mouse, bovine, rat, human, pig

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... NEFL(4747)

일반 설명

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

특이성

Recognizes the low molecular weight (68-70 kDa) subunit of the neurofilament triplet (NF-L), the epitope is phosphate independent. Reacts with human and higher vertebrates.

면역원

Enzymatically dephosphorylated pig neurofilaments.

애플리케이션

Immunoblot and immunohistochemistry (frozen sections, little reactivity on formalin-fixed paraffin embedded material). Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB1615

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
This Anti-Neurofilament 70 kDa Antibody, clone DA2 is validated for use in WB, IC, IH for the detection of Neurofilament 70 kDa.

결합

Replaces: 04-1112

물리적 형태

Liquid. Contains no preservative.

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Immunomicroscopy of neurofilaments in chromaffin cells of the adult bovine adrenal gland.
Yang, H Y, et al.
The Journal of Comparative Neurology, 371, 461-468 (1996)
Carbon filament implants promote axonal growth across the transected rat spinal cord.
Khan, T, et al.
Brain Research, 541, 139-145 (1991)
Monocular deprivation provokes alteration of the neuronal cytoskeleton in developing cat lateral geniculate nucleus.
Kevin R Duffy, Joanna E Slusar, Kevin R Duffy, Joanna E Slusar
Visual Neuroscience null
Specific human astrocyte subtype revealed by affinity purified GFAP antibody; unpurified serum cross-reacts with neurofilament-L in Alzheimer.
Middeldorp, J; van den Berge, SA; Aronica, E; Speijer, D; Hol, EM
Testing null
Renata Novotny et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(18), 5084-5093 (2016-05-06)
The aggregation of amyloid-β peptide (Aβ) in brain is an early event and hallmark of Alzheimer's disease (AD). We combined the advantages of in vitro and in vivo approaches to study cerebral β-amyloidosis by establishing a long-term hippocampal slice culture

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