MAB1476-I

Anti-Chymotrypsin (Hu Pancreas) Antibody

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: CHYMO10-104.10, monoclonal
• 분자량: calculated mol wt 28 kDa
• 종 반응성: human
• 포장: antibody small pack of 100 μg
• 기술: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• 동형: IgG1
• UniProt 수납 번호: P40313
• 배송 상태: dry ice
• 저장 온도: -10 to -25°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Chymotrypsin is a serine endopeptidase that is synthesized in the pancreatic acinar cells as chymotrypsinogen that is cleaved by trypsin to generate active chymotrypsin. Activation of chymotrypsinogen involves proteolytic cleavage at two sites. The resultant three chains are held together by five disulfide bonds. Chymotrypsin is reported to preferentially cleave peptide amide bonds where the carboxyl side of the amide bond (the P1 position) is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine). The aromatic ring in their sidechain that fits into a ′hydrophobic pocket′ (the S1 position) of the enzyme. Two predominant forms of chymotrypsin have been described and are designated as chymotrypsin A and B. They display high degree of homology but have significantly different proteolytic characteristics. The active site residues in chymotrypsin are far apart in the primary sequence but are brought together in a crevice formed between the two protein domains.

특이성

Clone CHYMO10-104.10 is a mouse monoclonal antibody that detects human Chymotrypsin.

면역원

Purified human pancreatic chymotrypsin.

애플리케이션

Anti-Chymotrypsin (Hu Pancreas), clone CHYMO10-104.10, Cat. No. MAB1476-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in ELISA and Immunohistochemistry (Paraffin).

ELISA: (Dilution is assay dependent)

Immunohistochemistry: 1:1000.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

물리적 형태

Purified mouse monoclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.

저장 및 안정성

Maintain at -20°C in convenient aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 L or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable