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Merck
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주요 문서

MAB1430

Sigma-Aldrich

Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12)

clone 24.12.8 (PHM-12), Chemicon®, from mouse

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

클론

24.12.8 (PHM-12), monoclonal

종 반응성

human

제조업체/상표

Chemicon®

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

동형

IgG1

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

BIOCHEMISTRY:

p I = 5.5

특이성

MAB1430 provides a marker for an antigen known to be important in glomerular cell-matrix interactions, and should prove useful in research into the mechanisms involved in renal pathology. This antibody reacts with glomerular and tubular basement membranes in kidney as well as basal lamina of capillaries. It also labels mesangial cells and matrix within the glomerulus as well as basement membrane structures in all organs examined (Gusterson et al., 1984).

면역원

Human glomeruli

애플리케이션

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12) validated for use in IH, IH(P).
The antibody is suitable for use on both frozen and protease digested paraffin processed tissue.

SUGGESTED USAGE

Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.

Optimal working dilutions must be determined by the end user.

물리적 형태

Format: Purified
Purified immunoglobulin presented as a liquid in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide.

저장 및 안정성

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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The American Journal of Pathology null
Invading squamous cell carcinoma can retain a basal lamina. An immunohistochemical study using a monoclonal antibody to type IV collagen.
Gusterson, B A, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 51, 82-87 (1984)
Production of monoclonal antibodies to fibronectin, type IV collagen and other antigens of the human glomerulus.
Hancock, W W, et al.
Pathology, 16, 197-206 (1984)
S E Aggrey et al.
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