MAB13406

Anti-MMP-2 Antibody, pro and active form, clone VB3

clone VB3, Chemicon^®, from mouse

• 동의어(들): Gelatinase A, 72 kDa Type IV Collagenase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: VB3, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• 동형: IgG1
• 적합성: not suitable for immunohistochemistry
• NCBI 수납 번호: NM_004530.2
• UniProt 수납 번호: P08253
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MMP2(4313)

설명

특이성

The antibody recognizes proteins of 72kDa and 66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72 kDa collagenase IV or gelatinase A). Shows no cross-reactivity with pro and active forms of other MMPs. MMPs are proteolytic enzymes capable of degrading connective tissue components. Degradation of the extracellular matrix (ECM) is an essential step in tumor invasion and metastasis. MMPs each have different substrate specificities within the ECM and are important in its degradation. MMP-2 mainly degrades type IV collagen and denatured collagens. MMP activity is modulated by tissue inhibitors of metalloproteinases (TIMP). Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by TIMP have been implicated in the invasive potential of malignant tumors.Cellular Localization: cytoplasmic

면역원

Epitope: pro and active form

Human native 72 kDa Gelatinase A.

애플리케이션

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-MMP-2 Antibody, pro & active form, clone VB3 is validated for use in ELISA, IF, WB for the detection of MMP-2.

Western blot: 1:200-1:400 for 2 hours at room temperature

ELISA

Immunofluorescence

Does not work for immunohistochemistry Optimal working dilutions must be determined by end user.

결합

Replaces: 04-1048

물리적 형태

200 μg/mL. of antibody purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Format: Purified

저장 및 안정성

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

분석 메모

Control
POSITIVE CONTROL: Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta. Bladder, breast and ovarian carcinomas.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable