추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
4B6, monoclonal
종 반응성
sheep
제조업체/상표
Chemicon®
기술
ELISA: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
sheep ... Il6(443406)
특이성
Reacts with ovine IL-6. No reactivity detected to recombinant ovine IL-1beta, IL-8, MCP or TNFalpha.
SPECIES REACTIVITY:
The antibody is likely to react with caprine (goat) IL-6.
SPECIES REACTIVITY:
The antibody is likely to react with caprine (goat) IL-6.
면역원
Recombinant ovine IL-6
애플리케이션
Capture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol.
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-6
1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-6
1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
This Anti-Interleukin-6 Antibody, clone 4B6 is validated for use in ELISA for the detection of Interleukin-6.
물리적 형태
Format: Purified
Liquid in PBS containing 0.1% sodium azide.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Systemic and cerebral inflammatory response to umbilical cord occlusions with worsening acidosis in the ovine fetus.
Andrew P Prout,Martin G Frasch,Ruud A W Veldhuizen,Rob Hammond,Michael G Ross,Bryan S Richardson
American Journal of Obstetrics and Gynecology null
Interleukin-1 in lipopolysaccharide induced chorioamnionitis in the fetal sheep.
Clare A Berry,Ilias Nitsos,Noah H Hillman,J Jane Pillow,Graeme R Polglase,Boris W Kramer et al.
Reproductive Sciences null
Andrew P Prout et al.
PloS one, 7(6), e39043-e39043 (2012-06-30)
Fetal hypoxic episodes may occur antepartum with the potential to induce systemic and cerebral inflammatory responses thereby contributing to brain injury. We hypothesized that intermittent umbilical cord occlusions (UCOs) of sufficient severity but without cumulative acidosis will lead to a
A Fernandez-Bustamante et al.
Respiratory physiology & neurobiology, 183(2), 149-158 (2012-06-26)
The regional distribution of inflammation during acute lung injury (ALI) is not well known. In an ovine ALI model we studied regional alveolar inflammation, surfactant composition, and CT-derived regional specific volume change (sVol) and specific compliance (sC). 18 ventilated adult
Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep.
Snyder, CC; Wolfe, KB; Gisslen, T; Knox, CL; Kemp, MW; Kramer, BW; Newnham, JP; Jobe et al.
American Journal of Obstetrics and Gynecology null
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