IHCR2025-6

IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3

clone AE1/AE3, from mouse

• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: AE1/AE3, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®; IHC Select
• 기술: immunohistochemistry: suitable (paraffin)
• 동형: IgG1
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... KRT1(3848)

설명

일반 설명

Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll, R., 1982). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50′, 48, 46, 45, and 40 kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kD. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun, T.T., 1984; Cooper, D., 1985; Sun, T. T., 1985 ). The 56.5/65-67 kD pair is present in keratinized (differentiated) epidermis. The 55/64 kD pair is characteristic of normal (corneal-type) epithelial differentiation (Moll, R., 1982; Sun, T.T, 1984). The 51/59 kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll, R., 1982; Cooper, D., 1985). The 51/58 kD pair is a keratinocyte marker; this pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll, R., 1982; Sun, T.T., 1984). The 48/56 kD pair is characteristic of hyper-proliferative (de-differentiated) keratinocytes (Moll, R., 1982; Weiss, R.A., 1984). The 45/52 kD pair and to a lesser extent, the 46/54 kD pair are characteristic of simple epithelia (Moll, R., 1982). The 40 kD keratin is present in most epithelia except adult epidermis (Moll, R., 1982).

특이성

Anti-Keratin AE1 recognizes the 56.5, 50, 50′, 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily. AE1/AE3 reacts with both acidic and basic keratins. Staining is localized to the cytoplasm. AE1/AE3 specifically binds to antigens located in the cytoplasm of normal epithelia. Reacts with cells of epithelial origin including simple and stratified epithelia and epidermis.

면역원

Human epidermal keratin

애플리케이션

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select Detection Kits.

Cytokeratin AE1/AE3 has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2025-6.

IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).

Research Category
Cell Structure

Research Sub Category
Cytokeratins

물리적 형태

Format: Purified

Purified monoclonal mouse antibody supplied in liquid format diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.

저장 및 안정성

Maintain at 2-8°C . Refer to vial for expiration dating.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317,H412
• 예방조치 성명서: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable