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Merck
모든 사진(1)

Key Documents

GF348

Sigma-Aldrich

TWEAK,
Human Recombinant Animal Free

동의어(들):

TNFSF12, MGC129581, DR3LG, TWEAK, APO3L

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About This Item

UNSPSC 코드:
12352202
eCl@ss:
32160405
NACRES:
NA.77

불순물

<0.1 μg/mg protein endotoxin (<1 EU/μg)

Quality Level

UniProt 수납 번호

배송 상태

dry ice

일반 설명

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
TWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NFkB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-. TWEAK also promotes proliferation and migration of endothelial cells.
Recombinant human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.

애플리케이션

Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

품질

Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ. The ED50 for this effect is between 30-45 ng/ml.

물리적 형태

Product is presented in 10 mM Sodium Phosphate, pH 7.5 + 80
mM L-Arginine and is filtered through a 0.2 micron filter before lyophilization.

저장 및 안정성

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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