GF310

PDGF-BB Protein, Human Recombinant Animal Free

The Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents.

• 동의어(들): Platelet Derived Growth Factor-BB, Platelet Derived Growth Factor, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 분석: >98% (SDS-PAGE and HPLC)
• 불순물: <0.1 μg/mg protein endotoxin (<1EU/μg)
• UniProt 수납 번호: P01127
• 배송 상태: dry ice

설명

일반 설명

Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the αgranules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Product Source: E. coli

특이성

Cross Reactivty
Cow, Monkey, Mouse, Pig, Rat

애플리케이션

Research Category
Inflammation & Immunology

Research Sub Category
Growth Factors & Receptors

품질

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.

물리적 형태

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents

저장 및 안정성

Store at -20°C for up to 6 months from date of receipt.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1