P4306

Platelet-Derived Growth Factor-BB human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): PDGF-BB
• MDL number: MFCD00213023
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 1.0-3.0 ng/mL EC₅₀
• 품질: endotoxin tested
• 분자량: 24.3 kDa
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg
• UniProt 수납 번호: P01127
• 저장 온도: −20°C
• 유전자 정보: human ... PDGFB(5155)

설명

일반 설명

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

애플리케이션

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

생화학적/생리학적 작용

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

기타 정보

Dimer of the B chain of human PDGF.

물리적 형태

Lyophilized from a 0.2 μm filtered solution.

분석 메모

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)