ECM660

Pseudopodia Purification Kit

The Pseudopodia Purification Kit is a novel system designed to specifically isolate & purify extending pseudopodia proteins from the cell body.

• UNSPSC 코드: 12352207
• eCl@ss: 32161000
• NACRES: NA.75

속성

• Quality Level: 100
• 제조업체/상표: Chemicon^®
• 기술: activity assay: suitable
• 응용 분야: sample preparation
• 배송 상태: wet ice

설명

일반 설명

Chemotaxis, or directed cell movement is a fundamental cellular process, necessary for embryonic development, angiogenesis, wound healing, and immune responses, as well as pathological events associated with inflammation and cancer metastasis [Lauffenburger & Horwitz, 1996]. Prior to cell body translocation or chemotaxis, a cell must first extend a dominant pseudopodium (lamellipodium) in the direction of the chemoattractant gradient [Lauffenburger & Horwitz, 1996; Parent & Devreotes, 1999]. Once a dominant pseudopodium is formed, cell movement can begin in the direction of the gradient. Formation of a directional pseudopodium is tightly controlled by signal transduction mechanisms that temporally and spatially regulate changes in focal adhesions, microtubules, and the actin/myosin cytoskeleton [Ridley et al., 1992; Schmidt et al., 1993; Manes et al., 1999; Nobes & Hall, 1999; Eddy et al., 2000].

The CHEMICON Pseudopodia Purification Kit is a novel system designed to specifically isolate and purify extending pseudopodia proteins from the cell body [Cho & Klemke, 2002]. This Kits provides a quick and efficient system to purify pseudopodia proteins and their responses to various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. This kit provides a novel method for pseudopodia protein purification; identify signaling complex formation as a result of chemotaxis stimuli.

For Research Use Only; Not for use in diagnostic procedures

애플리케이션

Research Category
Cell Structure

성분

Pseudopodia Purification Inserts - (Catalog No. 71608) 1 X 6 well plate containing 6 X 24mm 3.0-micrometer transwells.

Western Blot Lysis Buffer - (Catalog No. 71606) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% SDS

Immunoprecipitation Buffer - (Catalog No. 71601) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% Triton-X-100

Cell Scrapers - (Catalog No.71603) 3 each

Forceps - (Catalog No. 10203) 1 each

Cotton Swabs - (Catalog No. 10202) 14 swabs

저장 및 안정성

Store Kit and Materials at 2-8ºC until their expiration date

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319,H412
• 예방조치 성명서: P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable