추천 제품
생물학적 소스
goat
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
F(ab′)2 fragment of affinity isolated antibody
항체 생산 유형
secondary antibodies
클론
polyclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
ELISA: suitable
western blot: suitable
동형
IgG
배송 상태
wet ice
타겟 번역 후 변형
unmodified
관련 카테고리
일반 설명
IgG is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
특이성
Specific for human IgG, IgM and IgA.
애플리케이션
Goat anti-Human IgA Antibody, IgG & IgM, Alkaline Phosphatase detects level of Human IgA & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Western Blot:
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
품질
Routinely evaluated by Enzyme-linked Immunosorbent Assay.
ELISA:
1:5,000-1:50,000 diltution can be used.
ELISA:
1:5,000-1:50,000 diltution can be used.
물리적 형태
ImmunoAffinity Purified
Purified goat secondary antibody IgG in lyophilized buffer containing 0.01 M Tris-HCL, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.
저장 및 안정성
Store lyophilized material at 2-8°C for up to 1 year after date of receipt.
After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Dermal - Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Use of novel autoantibody and cancer-related protein arrays for the detection of esophageal adenocarcinoma in serum.
Arman Kilic,Matthew J Schuchert,James D Luketich,Rodney J Landreneau,Anna E Lokshin et al.
The Journal of Thoracic and Cardiovascular Surgery null
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