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Merck
모든 사진(2)

주요 문서

ABT59

Sigma-Aldrich

Anti-Pericentrin Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Pericentrin, Kendrin, Pericentrin-B

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

기술

immunocytochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... PCNT(5116)

일반 설명

Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.

특이성

Other homologies: Mouse (89% sequence homology). No homology to Rat was found.

면역원

KLH-conjugated linear peptide corresponding to human Pericentrin.

애플리케이션

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.
This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.

품질

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.

표적 설명

~250 kDa observed.
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Alyssa C Rezey et al.
Scientific reports, 9(1), 7555-7555 (2019-05-19)
Allergic asthma is characterized by airway smooth muscle layer thickening, which is largely attributed to cell division that requires the formation of centrosomes. Centrosomes play a pivotal role in regulating bipolar spindle formation and cell division. Before mitosis, centrosomes undergo
Anastasiya Klebanovych et al.
Cells, 11(3) (2022-02-16)
ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which mediates the ufmylation of proteins in response to

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