ABT119

Anti-CELSR1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Cadherin EGF LAG seven-pass G-type receptor 1, Cadherin family member 9, Flamingo homolog 2, hFmi2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology), human (based on 100% sequence homology)
• 기술: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_055061
• UniProt 수납 번호: Q9NYQ6
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CELSR1(9620)

설명

일반 설명

The Flamingo/CELSR family of 7TM-cadherins are characterized with a symmetric protein distribution and polarized activity at neighboring epithelial cell interfaces along defined axes of planar cell polarity. As a component of the extracellular matrix, the CELSR1 protein has been found particularly at the basal surface of neuroepithelial cells within both the early neural tube and a less well-defined group of ventricular zone cells at the midline of the developing spinal cord. CELSR1 plays a role in orienting hair follicles along the anterior–posterior axis of the developing mouse epidermis, both during embryogenesis and postnatal development.

면역원

Linear peptide corresponding to human CELSR1.

애플리케이션

Immunofluorescence Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected CELSR1 in rat cerebellum, substancia nigra, and hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected CELSR1 in neurons of rat cortex tissue and in Purkinje cells and cells within the granular layer of rat cerebellum tissue.

This Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.

품질

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CELSR1 in 10 µg of PC12 cell lysate.

표적 설명

~330 kDa observed. A second isoform at ~152 kDa may be observed in some lysates.
An uncharacterized band at ~54 kDa may be observed in some cell lysates.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable