ABN904
Anti-GAD 65/67
from rabbit, purified by affinity chromatography
동의어(들):
Glutamate decarboxylase 2/Glutamate decarboxylase 1, 65 kDa/67kDa glutamic acid decarboxylase, GAD-65/67, Glutamate decarboxylase 65/67 kDa isoform
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모든 사진(2)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat, mouse, human
포장
antibody small pack of 25 μg
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG
NCBI 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... GAD1(2571) , GAD2(2572)
일반 설명
Glutamate decarboxylase 2/Glutamate decarboxylase 1 (UniProt: Q05329/Q99259; also known as 65 kDa/67kDa glutamic acid decarboxylase, GAD-65/67, Glutamate decarboxylase 65/67 kDa isoform) is encoded by GAD2/GAD1 (also known as GAD65/GAD67) gene (Gene ID: 2572/2571) in human. Glutamate decarboxylase isoforms are expressed in the brain where it is involved in gamma-aminobutyric acid (GABA) synthesis. Glutamate decarboxylase 2 is also expressed in pancreatic tissue. Both GAD65 and GAD67 synthesize GABA at different locations in the cell and at different developmental stages. Usually, GAD1 is distributed evenly throughout the cell while isoform GAD2 is mainly localized to nerve terminals. GAD1 synthesizes GABA for neuronal activity that is related to as synaptogenesis and protection from neural injury. However, GAD2 isoform synthesizes GABA for neurotransmission activity.
특이성
This rabbit polyclonal antibody detects GAD2 and GAD1 in human, Mouse, and Rat. It targets an epitope within 14 amino acids from the C-terminal region.
면역원
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human glutamate decarboxylase 2 (GAD2).
애플리케이션
Anti-GAD 65/67 Antibody, Cat. No. ABN904, is a highly specific rabbit polyclonal antibody that targets Human, Mouse, and Rat GAD2 and GAD1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GAD 65/67 in human cerebellum, mouse cerebral cortex, and rat cerebral cortex tissues.
Research Category
Neuroscience
Neuroscience
품질
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected GAD 65/67 in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected GAD 65/67 in 10 µg of human brain tissue lysate.
표적 설명
~60/62kDa observed; 65.41/66.90 kDa calculated, respectively for GAD2 and GAD1. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
K P Briski et al.
Brain structure & function, 226(4), 1053-1065 (2021-02-14)
Recurring insulin-induced hypoglycemia (RIIH) in males correlates with maladaptive glucose counter-regulatory collapse and acclimated expression of ventromedial hypothalamic nucleus (VMN) nitric oxide (NO) and γ-aminobutyric acid (GABA) metabolic transmitter biomarkers, e.g., neuronal nitric oxide synthase (nNOS) and glutamate decarboxylase65/67 (GAD).
Md Main Uddin et al.
ASN neuro, 13, 17590914211035020-17590914211035020 (2021-10-02)
Brain glycogen is remodeled during metabolic homeostasis and provides oxidizable L-lactate equivalents. Brain glycogen phosphorylase (GP)-brain (GPbb; AMP-sensitive) and -muscle (GPmm; norepinephrine-sensitive) type isoforms facilitate stimulus-specific control of glycogen disassembly. Here, a whole animal model involving stereotactic-targeted delivery of GPmm
Karen P Briski et al.
Acta neurobiologiae experimentalis, 80(1), 57-65 (2020-03-28)
The catecholamine norepinephrine (NE) links hindbrain metabolic‑sensory neurons with downstream gluco‑regulatory loci, including the ventromedial hypothalamic nucleus (VMN). Exogenous NE up‑regulates VMN expression of glutamate decarboxylase (GAD), biomarker for the gluco‑inhibitory transmitter γ‑aminobutryic acid (GABA). Brain glycogen phosphorylase (GP)‑muscle (GPmm)
Abdulrahman Alhamyani et al.
AIMS neuroscience, 8(4), 510-525 (2021-12-09)
Gamma-aminobutyric acid (GABA) acts on ventromedial hypothalamic targets to suppress counter-regulatory hormone release, thereby lowering blood glucose. Maladaptive up-regulation of GABA signaling is implicated in impaired counter-regulatory outflow during recurring insulin-induced hypoglycemia (RIIH). Ventromedial hypothalamic nucleus (VMN) GABAergic neurons express
Elena N Judd et al.
eLife, 10 (2021-09-21)
The cerebellum consists of parallel circuit modules that contribute to diverse behaviors, spanning motor to cognitive. Recent work employing cell-type-specific tracing has identified circumscribed output channels of the cerebellar nuclei (CbN) that could confer tight functional specificity. These studies have
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