ABN368

Anti-Spastin Antibody

serum, from rabbit

• 동의어(들): Spastin, Spastic paraplegia 4 protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, rat, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_055761
• UniProt 수납 번호: Q9UBP0
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SPAST(6683)

설명

일반 설명

Spastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.

면역원

Recombinant protein corresponding to human Spastin with 4 isoforms.

애플리케이션

Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.

Western Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).

품질

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 µg of L6 cell lysate.

표적 설명

~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1