추천 제품
제품명
Anti-alpha-Synuclein Antibody, oligomer-specific Syn33, from rabbit
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human
포장
antibody small pack of 25 μg
기술
ELISA: suitable
immunofluorescence: suitable
inhibition assay: suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... SNCA(6622)
일반 설명
Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson′s disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein.
특이성
This rabbit polyclonal antibody specifically recognizes aggregated form of alpha-synuclein.
면역원
Synuclein oligomers from full length human wild-type alpha-synuclein.
애플리케이션
Anti-alpha-Synuclein, oligomer-specific Syn33 Antibody, Cat. No. ABN2265, is a highly specific rabbit polyclonal antibody that targets aggregted alpha-synuclein and has been tested in ELISA, Inhibition, Immunofluorescence, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected alpha-Synuclein, oligomers in brain cortices from Parkinson s disease (PD) and dementia with Lewy bodies (DLB) patients (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomers. (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomer. (Courtesy of Dr. Rakez Kayed′s laboratory at University of Texas Medical Branch, Galveston).
ELISA Analysis: A representative lot detected alpha-Synuclein, oligomers in ELISA application (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Inhibition Analysis: A representative lot inhibited cytotoxicity exerted alpha-synuclein oligomers in human neuroblastoma SH-SY5Y cells measured by MTT-based assay (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomers. (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomer. (Courtesy of Dr. Rakez Kayed′s laboratory at University of Texas Medical Branch, Galveston).
ELISA Analysis: A representative lot detected alpha-Synuclein, oligomers in ELISA application (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Inhibition Analysis: A representative lot inhibited cytotoxicity exerted alpha-synuclein oligomers in human neuroblastoma SH-SY5Y cells measured by MTT-based assay (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
품질
Evaluated by Western Blotting in Alpha Synuclein aggregate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in alpha Synuclein aggregates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in alpha Synuclein aggregates.
물리적 형태
Format: Purified
Protein A purified
Purified rabbit polyclonal antibody in PBS.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Kenya Moore et al.
Molecular neurobiology, 1-15 (2023-01-28)
The pathological hallmark of many neurodegenerative diseases is the accumulation of characteristic proteinaceous aggregates. Parkinson's disease and dementia with Lewy bodies can be characterized as synucleinopathies due to the abnormal accumulation of the protein alpha-synuclein (α-Syn). Studies have shown amyloidogenic
A R Esteves et al.
Scientific reports, 10(1), 13149-13149 (2020-08-06)
Many hypotheses have been postulated to define the etiology of sporadic Parkinson's and Alzheimer's disorders (PD and AD) but there is no consensus on what causes these devastating age-related diseases. Braak staging of both pathologies helped researchers to better understand
Philip Wing-Lok Ho et al.
Autophagy, 16(2), 347-370 (2019-04-16)
Parkinson disease (PD) is an age-related neurodegenerative disorder associated with misfolded SNCA/α-synuclein accumulation in brain. Impaired catabolism of SNCA potentiates formation of its toxic oligomers. LRRK2 (leucine-rich repeat kinase-2) mutations predispose to familial and sporadic PD. Mutant LRRK2 perturbs chaperone-mediated-autophagy
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