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Merck
모든 사진(2)

주요 문서

ABE316

Sigma-Aldrich

Anti-ARID1B Antibody

from rabbit, purified by affinity chromatography

동의어(들):

AT-rich interactive domain-containing protein 1B, ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

기술

immunoprecipitation (IP): suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... ARID1B(57492)

일반 설명

ARID1B (AT rich interactive domain 1B), also known as BAF250B, p250R and hOsa2, is a nuclear protein and a member of the SWI/SNF-A chromatin-remodeling complex. It is involved in repression of select genes by chromatin remodeling and transcriptional activation. It non-specifically binds DNA and alters DNA nucleosome topology. The cause of mental retardation autosomal dominant type 12 (MRD12) are defects in ARID1B. High levels of ARID1B can be found in heart, skeletal muscle and kidney.

면역원

KLH-conjugated linear peptide corresponding to human ARID1B.

애플리케이션

Immunoprecipitation Analysis: A representative lot immunoprecipitated ARID1B from Raji cell nuclear extract.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
This Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.

품질

Evaluated by Western Blot in Raji cell nuclear extract.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ARID1B in 10 µg of Raji cell nuclear extract.

표적 설명

~250 kDa observed. Uncharacterized bands may be observed at ~16 kDa and ~32 kDa in some lysates.. Uniprot describes 4 isoforms produced by alternative splicing at ~236 kDa, ~237 kDa, ~241 kDa and ~162 kDa This antibody will recognize all isoforms.

물리적 형태

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Raji cell nuclear extract

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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