ABE21

Anti-Smad4/DPC4 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, rat, human
• 종 반응성(상동성에 의해 예측): chimpanzee (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_005350.1
• UniProt 수납 번호: Q13485
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SMAD4(4089)

설명

일반 설명

SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors.

특이성

This antibody recognizes Smad4 at the SAD region.

면역원

Epitope: SAD region

GST-tagged recombinant protein corresponding to human Smad4 at the SAD region.

애플리케이션

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Smad4 in NIH/3T3, HeLa, and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Smad4/DPC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Smad4/DPC4 also known as Mothers against decapentaplegic homolog 4, SMAD 4, Smad4, hSMAD4.

품질

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Smad4 on 10 µg of HepG2 cell lysate.

표적 설명

~68 kDa observed.

결합

Replaces: 04-1033

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HepG2 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable