ABE1363

Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Histone H2A.Z, H2A/z
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: ChIP; DB; ELISA; ICC; WB
• 종 반응성: mouse, human
• 기술: ChIP: suitable (ChIP-seq); ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 7

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 기술: ChIP: suitable (ChIP-seq); ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_002097
• UniProt 수납 번호: P0C0S5
• 배송 상태: wet ice
• 타겟 번역 후 변형: acetylation (Lys5,Lys7,Lys11)
• 유전자 정보: human ... H2AFZ(3015)

설명

일반 설명

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

특이성

Human, mouse, wide range expected.

면역원

KLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus.

애플리케이션

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.

This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11).

품질

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract.

표적 설명

~16 kDa observed

물리적 형태

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 0.05% ProClin300.

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable