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Merck
모든 사진(4)

주요 문서

ABE1321

Sigma-Aldrich

Anti-MBD6

from rabbit, purified by affinity chromatography

동의어(들):

Methyl-CpG-binding domain protein 6, Methyl-CpG-binding protein MBD6

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

mouse, human

포장

antibody small pack of 25 μg

기술

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

타겟 번역 후 변형

unmodified

유전자 정보

human ... MBD6(114785)

관련 카테고리

일반 설명

Methyl-CpG-binding domain protein 6 (UniProt: Q96DN6; also known as Methyl-CpG-binding protein MBD6) is encoded by the MBD6 (also known as KIAA1887) gene (Gene ID: 114785) in human. The methyl-CpG-binding domain (MBD) proteins are primary candidates for the readout of DNA methylation. MBD proteins require a functional MBD domain and methyl-CpGs, although some MBD proteins can also bind unmethylated DNA. MBD proteins are involved in transcriptional repression by histone modification, nucleosome remodeling or chromatin organization. MBD6 is highly expressed in testes. Its MBD domain is localized in the N-terminal region (aa 11-81) and it also contains proline-rich domains. MBD6 binds to heterochromatin, but does not interact with either methylated or unmethylated DNA. MBD6 is reported to interact directly with the human polycomb deubiquitinase complex, PR-DUB, which catalyzes deubiquitination of H2AK119. Mutations in MBD6 that lead to R883W alteration are reported to cause autism. (Ref.: Cukier, HN., et al. (2012). Autism Res. 5(6); 385-397; Du, Q., et al. (2015). Epigenomics 7(6); 1051-1073).

특이성

This rabbit polyclonal antibody detects Methyl-CpG-binding domain protein 6 in human and murine cells. It targets an epitope within 16 amino acids from the N-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Methyl-CpG-binding domain protein 6.

애플리케이션

Anti-MBD6, Cat. No. ABE1321, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 6 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD6 in HeLa, A431, HEK293, and NIH3T3 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD6 in human skin and human stomach tissue sections.
Research Category
Epigenetics & Nuclear Function

품질

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MBD6 in SH-SY5Y cell lysate.

표적 설명

~110 kDa observed; 101.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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