ABD119

Anti-TLE1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_005068
• UniProt 수납 번호: Q04724
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TLE1(7088)

설명

일반 설명

Transducin-like enhancer protein 1 (UniProt Q04724; also known as E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1) is encoded by the TLE1 gene (Gene ID 7088) in human. TLE proteins interact with HDACs as well as deacetylated histone tails and play an important role in transcription regulation in several signaling pathways, including Ras, Notch, and Wnt. TLE proteins contain a conserved N-terminal glutamine-rich (Q) domain (a.a. 1-131 of TLE1) that mediates TLEs tetramerization as well as TCF/LEF proteins binding, and is essential for chromatin oligomerization. This is followed by a variable central domain sub-divided into a glycine-prolin-rich (GP) region (a.a. 132-199 of TLE1), the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain (a.a. 200-268), a serine-proline-rich (SP) region (a.a. 200-268 and 269-450 of TLE1), and a C-terminal WD40 domain with 6 WD repeats (a.a. 470-767 of TLE1) that interact with multiple transcription factors. The GP region binds directly to HDAC1, the CcN region contains consensus CK2 and Cdc2 phosphorylation sites that are modified in a cell cycle-dependent manner, the SP region is phosphorylated by the Ser/Thr kinases HIPK2 and MAPK, which appear to negatively regulate transcription repression activity, and may also interact with basic helix-loop-helix transcription factors. The C-terminal WD40 domain is essential for chromatin condensation and, together with SP, interacts with bHLH proteins.

특이성

This polyclonal antibody targets the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain of TLE1 and stained cell nuclei by fluorescent immunocytochemistry.

면역원

Epitope: CcN domain.

KLH-conjugated linear peptide corresponding to a sequence within the CcN domain of human TLE1.

애플리케이션

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected TLE1 in HeLa cells and H9 human stem cells.

Research Category
Stem Cell Research

Research Sub Category
Histone Modifying Proteins

This Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.

품질

Evaluated by Western Blotting in H9 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antiobdy detected TLE1 in 10 µg of H9 human stem cell lysate.

표적 설명

~90 kDa observed. 83.20 kDa calculated.

물리적 형태

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1