추천 제품
생물학적 소스
goat
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, bovine
제조업체/상표
Chemicon®
기술
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
적합성
not suitable for immunohistochemistry (Paraffin)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... COL1A1(1277)
특이성
Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
면역원
Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
애플리케이션
Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Dot and slot blotting: 1:100-1:500
ELISA: 1:50-1:100
Indirect immunohistochemistry (frozen sections only): 1:10-1:20
Indirect immunocytochemistry: 1:10-1:20
Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
ELISA: 1:50-1:100
Indirect immunohistochemistry (frozen sections only): 1:10-1:20
Indirect immunocytochemistry: 1:10-1:20
Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
물리적 형태
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
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신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
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Acta Histochemica null
I Durán et al.
Developmental biology, 354(1), 160-172 (2011-03-23)
The skeleton of zebrafish fins consists of lepidotrichia and actinotrichia. Actinotrichia are fibrils located at the tip of each lepidotrichia and play a morphogenetic role in fin formation. Actinotrichia are formed by collagens associated with non-collagen components. The non-collagen components
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