추천 제품
제품명
Anti-Rat Collagen Type I Antibody, Chemicon®, from rabbit
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
동형
IgG
적합성
not suitable for Western blot
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... COL1A1(1277)
일반 설명
Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.
특이성
Recognizes Rat Collagen Type I
The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.
면역원
Collagen Type I extracted and purified from rat skin.
애플리케이션
Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in RIA.
Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.
Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.
Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.
A 1:200 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in RIA.
Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.
Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.
Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.
품질
Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.
IHC-Paraffin Staining With Epitope Retrieval: Rat Skin
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.
IHC-Paraffin Staining With Epitope Retrieval: Rat Skin
물리적 형태
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.
분석 메모
Control
Rat hepatic stellate cell lysate
Rat hepatic stellate cell lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
CCN3/CCN2 regulation and the fibrosis of diabetic renal disease.
Riser BL, Najmabadi F, Perbal B, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC, Peterson DR
Journal of Cell Communication and Signaling null
Thy-1 expression by cardiac fibroblasts: lack of association with myofibroblast contractile markers.
Francois Hudon-David,Fatiha Bouzeghrane,Patrick Couture,Gaetan Thibault
Journal of Molecular and Cellular Cardiology null
Intraglomerular pressure and mesangial stretching stimulate extracellular matrix formation in the rat.
Riser, B L, et al.
The Journal of Clinical Investigation, 90, 1932-1943 (1992)
Partial hepatectomy-induced regeneration accelerates reversion of liver fibrosis involving participation of hepatic stellate cells.
Suarez-Cuenca, Juan A, et al.
Exp. Biol. Med, 233, 827-839 (2008)
Alan S Liu et al.
Scientific reports, 6, 33919-33919 (2016-09-28)
The biomechanical behavior of tissues under mechanical stimulation is critically important to physiological function. We report a combined experimental and modeling study of bioengineered 3D smooth muscle microtissues that reveals a previously unappreciated interaction between active cell mechanics and the
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