AB5935

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

Chemicon^®, from rabbit

• 동의어(들): Tyrosine Monooxygenase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: avian, rat, mouse
• 종 반응성(상동성에 의해 예측): quail (100% sequence homology), nonhuman primates (100% sequence homology), human (100% sequence homology), porcine (100% sequence homology)
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NM_000360.3
• UniProt 수납 번호: P07101
• 배송 상태: dry ice
• 타겟 번역 후 변형: phosphorylation (pSer40)
• 유전자 정보: human ... TH(7054) ; rat ... Th(25085)

설명

일반 설명

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

특이성

Other species have not been tested.

Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.

면역원

Epitope: phospho-Ser40

Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.

애플리케이션

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.

Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

품질

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.

표적 설명

60 kDa

물리적 형태

ImmunoAffinity Purified

Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.

저장 및 안정성

Stable for 6 months at -20°C in undiluted from date of receipt.

분석 메모

Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2