생물학적 소스
rabbit
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
mouse, rat
제조업체/상표
Chemicon®
기술
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... VAMP2(6844)
특이성
Recognizes VAMP-2 (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2). The epitope does not share homology with any other known proteins.
SPECIES REACTIVITIES: It is expected that the antibody will also react with human, Macaca multata and bovine due to sequence homology (25/26). Other species have not been tested.
SPECIES REACTIVITIES: It is expected that the antibody will also react with human, Macaca multata and bovine due to sequence homology (25/26). Other species have not been tested.
면역원
GST fusion protein corresponding to a cytoplasmic, N-terminal part of rat or mouse VAMP-2 (Accession numbers Q64357, NP 036795).
애플리케이션
Anti-VAMP-2 Antibody detects level of VAMP-2 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Western blot: 1:1,000 using ECL on rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
물리적 형태
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min)
저장 및 안정성
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
분석 메모
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Helmut Kubista et al.
Journal of cell science, 117(Pt 6), 955-966 (2004-02-06)
The core complex, formed by the SNARE proteins synaptobrevin 2, syntaxin 1 and SNAP-25, is an important component of the synaptic fusion machinery and shows remarkable in vitro stability, as exemplified by its SDS-resistance. In western blots, antibodies against one
Kwok-Ho Lam et al.
Cell reports, 30(8), 2526-2539 (2020-02-27)
Botulinum neurotoxin (BoNT) is one of the most acutely lethal toxins known to humans, and effective treatment for BoNT intoxication is urgently needed. Single-domain antibodies (VHH) have been examined as a countermeasure for BoNT because of their high stability and
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